- The Returners: Season One Omnibus?
- Addis Ababa University Libraries catalog › Details for: Antisense research and application /?
- Mollys Revenge (Serenity Inn Series Book 7).
- Antisense Research and Application (Handbook of Experimental Pharmacology).
Get Access Get Access. Methods in Enzymology Volume , , Pages Author links open overlay panel Stanley T.
- Satans Diary?
- Unintrigante offerta di lavoro (Italian Edition).
- Oligonucleotide-based drugs in the control of cytokine synthesis.
- Sutcliffes Commentary on the Old & New Testaments - Book of Lamentations;
- Progress in antisense technology: The end of the beginning - ScienceDirect;
- Growing Up Stories - True Stories of a Brown Dirt Boy;
- Playing with Fire.
Publisher Summary During the past decade, intense efforts to develop and exploit antisense technology have been mounted. Recommended articles Citing articles 0.
To better understand the metabolism of these agents, we studied the stability of several phosphorothioate oligodeoxynucleotides, their congeners, and second generation oligomer chemistries in rat liver homogenates. To examine metabolism, background nuclease activity was characterized in whole liver homogenates by using ISIS , a mer phosphodiester oligodeoxynucleotide. Nuclease activity could readily be detected in liver homogenates. Our data indicate that metabolism of phosphorothioate oligodeoxynucleotides was more complex than that of phosphodiesters for many reasons, including phosphorothioate oligodeoxynucleotide inhibition of nucleases and the presence of R p and S p stereoisomers.
Antisense Research and Application | Stanley T. Crooke | Springer
The rate of phosphorothioate metabolism also appeared to be influenced by sequence, with pyrimidine-rich compounds being metabolized to a greater extent than purine-rich oligomers. Other factors affecting stability included oligomer chemistry and length. Concomitant experiments performed in rats dosed systemically with the same compounds mimic the activities seen in vitro and suggest that this liver homogenate system is a valuable model with which to study the mechanism of metabolism of antisense oligonucleotides.
Send reprint requests to: Dr.
Rosanne M. Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail.
Antisense Therapy: An Overview
We do not retain these email addresses. Skip to main content. Crooke , Mark J. Graham , Michael J. Martin , Kristina M.
You are here
Lemonidis , Tad Wyrzykiewiecz and Lendell L. Journal of Pharmacology and Experimental Therapeutics January , 1 ;.
Abstract Phosphorothioate antisense oligodeoxynucleotides are novel therapeutic agents designed to selectively and specifically inhibit production of various disease-related gene products.